Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
The authors gratefully acknowledge funding from the National Institutes of Health (Grant Number R00GM081450 ), the National Academies Keck Futures Initiative (Grant Number NAFKI-SB5 ), the National Science Foundation (Grant Number MCB-0943393 ), the Office of Naval Research (Grant Number N00014-11-1-0363 ), the DARPA YFA Program (Grant Number N66001-11-1-4137 ), and the ARMY Research Office (Grant Number W911NF-11-1-044 ).
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abstract = "Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.",
keywords = "Cell-free biology, Cell-free protein synthesis, E. coli extract, High-throughput, In vitro translation, Personalized medicines, Recombinant DNA protein production, Synthetic biology, Wheat germ extract",
author = "Carlson,note = "Funding Information: The authors gratefully acknowledge funding from the National Institutes of Health (Grant Number R00GM081450 ), the National Academies Keck Futures Initiative (Grant Number NAFKI-SB5 ), the National Science Foundation (Grant Number MCB-0943393 ), the Office of Naval Research (Grant Number N00014-11-1-0363 ), the DARPA YFA Program (Grant Number N66001-11-1-4137 ), and the ARMY Research Office (Grant Number W911NF-11-1-044 ). ",
year = "2012", month = sep, doi = "10.1016/j.biotechadv.2011.09.016", language = "English (US)", volume = "30", pages = "1185--1194", journal = "Biotechnology Advances", issn = "0734-9750", publisher = "Elsevier Inc.",Research output : Contribution to journal › Review article › peer-review
T1 - Cell-free protein synthesis
T2 - Applications come of age
AU - Carlson, Erik D.
AU - Hodgman, C. Eric
AU - Jewett, Michael Christopher
N1 - Funding Information: The authors gratefully acknowledge funding from the National Institutes of Health (Grant Number R00GM081450 ), the National Academies Keck Futures Initiative (Grant Number NAFKI-SB5 ), the National Science Foundation (Grant Number MCB-0943393 ), the Office of Naval Research (Grant Number N00014-11-1-0363 ), the DARPA YFA Program (Grant Number N66001-11-1-4137 ), and the ARMY Research Office (Grant Number W911NF-11-1-044 ).
N2 - Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
AB - Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
KW - Cell-free biology
KW - Cell-free protein synthesis
KW - E. coli extract
KW - In vitro translation
KW - Personalized medicines
KW - Recombinant DNA protein production
KW - Synthetic biology
KW - Wheat germ extract